Correct for hydroxyproline in elastin when measuring collagen in tissues with a high elastin content.
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چکیده
منابع مشابه
The determination of collagen and elastin in tissues.
In the study of the occurrence and distribution of collagen in normal and pathological tissue and its formation and development in the embryo there has been need for aq accurate method to determine this substance in small amounts of tissue. Spencer, Morgulis, and Wilder (1) devised a method based on conversion of collagen to gelatin followed by the determination of the nitrogen of the gelatin p...
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PROCOLLAGEN-COLLAGEN CONVERSION The first extracellular step after secretion is the proteolytic cleavage of these Nand C-terminal nonhelical regions of procollagen (Bornstein, 1974). The actual location of cleavage of these extension peptides is not known, but they are probably removed during the formation of the fibre and may take part in fibrogenesis. The intermediates in the conversion of pr...
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Most abundant in the extracellular matrix are collagens, joined by elastin that confers elastic recoil to the lung, aorta, and skin. These fibrils are highly resistant to proteolysis but can succumb to a minority of the matrix metalloproteinases (MMPs). Considerable inroads to understanding how such MMPs move to the susceptible sites in collagen and then unwind the triple helix of collagen mono...
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SD Kucich et al. (1) described an enzyme-linked immunosorbent assay (EUSA) for soluble degradation products of elastin (elastin-derived peptides, EDP) in plasma. They reported a nonspecific loss of measurable EDP owing to coacervation of the elastin-a physical phenomenon in which certain proteins exhibit reversible aggregation on heating or increasing the ionic strength (2). We modified an ELIS...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1982
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/28.7.1709